A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria

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• 作者：Qingling Xu ; Cheol Ho Heo ; Jin A. Kim ; Hye Sue Lee ; Ying Hu ; Dayoung Kim ; Kunemadihalli Mathada Kotraiah Swamy ; Gyoungmi Kim ; Sang-Jip Nam ; Hwan Myung Kim ; Juyoung Yoon
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 21, 2016
• 年：2016
• 卷：88
• 期：12
• 页码：6615-6620
• 全文大小：365K
• 年卷期：0
• ISSN：1520-6882

文摘

Hypochlorite (OCl^–) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl^– at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl^– recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl^– in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl^–, displayed a selective fluorescent off/on response to OCl^– with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl^– in live RAW 264.7 cells via two-photon microscopy.