Mitochondria-Targeted Reaction-Based Fluorescent Probe for Hydrogen Sulfide

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• 作者：Yen Leng Pak ; Jun Li ; Kyoung Chul Ko ; Gyoungmi Kim ; Jin Yong Lee ; Juyoung Yoon
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：May 17, 2016
• 年：2016
• 卷：88
• 期：10
• 页码：5476-5481
• 全文大小：470K
• 年卷期：0
• ISSN：1520-6882

文摘

In this study, we developed a turn-on mitochondria-targeting hydrogen sulfide, “probe 1”, based on the selective thiolysis of 7-nitro-1,2,3-benzoxadiazole amine moiety attached to the piperazine-based naphthalimide scaffold. Probe 1 exhibited excellent properties with 68-fold fluorescence enhancement, a low detection limit (2.46 μM), a low cytotoxicity, and a good selectivity toward hydrogen sulfide. The success of intracellular imaging indicated that probe 1 could be used in further applications for the investigation of biological functions and pathological roles of H₂S in living systems.