A Synthetic Thermosensitive Hydrogel for Cartilage Bioprinting and Its Biofunctionalization with Polysaccharides

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• 作者：Anna Abbadessa ; Vivian H. M. Mouser ; Maarten M. Blokzijl ; Debby Gawlitta ; Wouter J. A. Dhert ; Wim E. Hennink ; Jos Malda ; Tina Vermonden
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：June 13, 2016
• 年：2016
• 卷：17
• 期：6
• 页码：2137-2147
• 全文大小：595K
• 年卷期：0
• ISSN：1526-4602

文摘

Hydrogels based on triblock copolymers of polyethylene glycol and partially methacrylated poly[N-(2-hydroxypropyl) methacrylamide mono/dilactate] make up an attractive class of biomaterials because of their biodegradability, cytocompatibility, and tunable thermoresponsive and mechanical properties. If these properties are fine-tuned, the hydrogels can be three-dimensionally bioprinted, to generate, for instance, constructs for cartilage repair. This study investigated whether hydrogels based on the polymer mentioned above with a 10% degree of methacrylation (M₁₀P₁₀) support cartilage formation by chondrocytes and whether the incorporation of methacrylated chondroitin sulfate (CSMA) or methacrylated hyaluronic acid (HAMA) can improve the mechanical properties, long-term stability, and printability. Chondrocyte-laden M₁₀P₁₀ hydrogels were cultured for 42 days to evaluate chondrogenesis. M₁₀P₁₀ hydrogels with or without polysaccharides were evaluated for their mechanical properties (before and after UV photo-cross-linking), degradation kinetics, and printability. Extensive cartilage matrix production occurred in M₁₀P₁₀ hydrogels, highlighting their potential for cartilage repair strategies. The incorporation of polysaccharides increased the storage modulus of polymer mixtures and decreased the degradation kinetics in cross-linked hydrogels. Addition of HAMA to M₁₀P₁₀ hydrogels improved printability and resulted in three-dimensional constructs with excellent cell viability. Hence, this novel combination of M₁₀P₁₀ with HAMA forms an interesting class of hydrogels for cartilage bioprinting.