Development of Porous Beads to Provide Regulated BMP-2 Stimulation for Varying Durations: In Vitro and In Vivo Studies for Bone Regeneration

详细信息    查看全文

• 作者：Ho Yong Kim ; Jin Ho Lee ; Jeong-Won Yun ; Jin-Ho Park ; Bong-Wook Park ; Gyu-Jin Rho ; Si-Jung Jang ; Ji-Sung Park ; Hee-Chun Lee ; Young Min Yoon ; Tae Sung Hwang ; Dong Hoon Lee ; June-Ho Byun ; Se Heang Oh
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：May 9, 2016
• 年：2016
• 卷：17
• 期：5
• 页码：1633-1642
• 全文大小：737K
• 年卷期：0
• ISSN：1526-4602

文摘

It is commonly accepted that the sustained release of bone morphogenetic protein-2 (BMP-2) can enhance bone regeneration and minimize its safety issues. However, little is known regarding the appropriate duration of BMP-2 stimulation for sufficient osteogenic differentiation and new bone formation because of the short half-life of BMP-2 in the physiological environment and the lack of a well-defined delivery matrix that can regulate the release period of BMP-2. In this study, we prepared porous poly(lactic-co-glycolic acid) (PLGA) beads with different surface pore sizes that can regulate the release period of BMP-2 (i.e., 7, 17, and 30 days) while providing the BMP-2 concentration required for bone regeneration. Our findings in both in vitro cell culture and in vivo animal studies using these BMP-2-loaded beads demonstrate that release of BMP-2 within 7 days affects only the initial differentiation of human periosteum-derived cells (hPDCs) and does not significantly enhance their subsequent differentiation into mature functional cells. However, extending the duration of BMP-2 stimulation over 17 days can provide a suitable environment for osteogenic differentiation of hPDCs and new bone formation.