Anti-Food Allergic Activity of Sulfated Polysaccharide from Gracilaria lemaneiformis is Dependent on Immunosuppression and Inhibition of p38 MAPK

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• 作者：Qing-Mei Liu ; Yang Yang ; Soheila J. Maleki ; Marcos Alcocer ; Sha-Sha Xu ; Chao-Lan Shi ; Min-Jie Cao ; Guang-Ming Liu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：June 8, 2016
• 年：2016
• 卷：64
• 期：22
• 页码：4536-4544
• 全文大小：535K
• 年卷期：0
• ISSN：1520-5118

文摘

Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extraction and ethanol precipitation followed by column chromatography. BALB/c mice, RBL-2H3, and KU812 cells were used for verifying the anti food allergic activity of GLSP. According to the results of mice experiment, GLSP was able to alleviate allergy symptoms, to reduce TM-specific IgE and IgG1, to suppress Th2 cell polarization, and to promote the function of regulatory T (Treg) cells. In addition, GLSP had the ability to inhibit the function of RBL-2H3 cells. Furthermore, GLSP inhibited the activation of KU812 via suppression of p38 mitogen-activated protein kinase (MAPK). In conclusion, immunosuppression as well as the reduction in the level of p38 MAPK may contribute to GLSP’s putative activity against food allergy. GLSP may be used as a functional food component for allergic patients.