Effectiveness of Periodic Treatment of Quercetin against Influenza A Virus H1N1 through Modulation of Protein Expression

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• 作者：Bipin Vaidya ; Se-Young Cho ; Kyung-Seo Oh ; Song Hak Kim ; Yeong O Kim ; Eun-Hye Jeong ; Thoa Thi Nguyen ; Sung Hyun Kim ; In Seon Kim ; Joseph Kwon ; Duwoon Kim
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：June 1, 2016
• 年：2016
• 卷：64
• 期：21
• 页码：4416-4425
• 全文大小：527K
• 年卷期：0
• ISSN：1520-5118

文摘

Kimchi, a traditional fermented food regularly consumed in Korea, contains various types of antimicrobial compounds. Among the tested compounds present in common spices used in Kimchi, quercetin showed the highest selectivity index against influenza A virus (IAV) H1N1. In this study, the effect of pretreatment and periodic treatment with quercetin against IAV in Madin–Darby canine kidney cells was observed. Compared to pretreatment, periodic treatment resulted in significantly higher cell viability but lower relative expression of the IAV PA gene and total apoptosis and cell death. To explain the mechanisms underlying the antiviral effects of quercetin treatment, a comparative proteomic analysis was performed in four samples (mock, quercetin-treated, IAV-infected, and quercetin-treated IAV-infected). Among the 220 proteins, 56 proteins were classified nonhierarchically into three clusters and were differentially modulated by quercetin treatment in IAV-infected cells. Post-translational modifications were identified in 68 proteins. In conclusion, periodic treatment with quercetin is effective in reducing IAV infection, and differentially regulates the expression of key proteins, including heat shock proteins, fibronectin 1, and prohibitin to reduce IAV replication.