Antiallergic Activity of Ethanol Extracts of Arctium lappa L. Undried Roots and Its Active Compound, Oleamide, in Regulating FcεRI-Mediated and MAPK Signaling in RBL-2H3 Cells

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• 作者：Woong-Suk Yang ; Sung Ryul Lee ; Yong Joon Jeong ; Dae Won Park ; Young Mi Cho ; Hae Mi Joo ; Inhye Kim ; Young-Bae Seu ; Eun-Hwa Sohn ; Se Chan Kang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：May 11, 2016
• 年：2016
• 卷：64
• 期：18
• 页码：3564-3573
• 全文大小：464K
• 年卷期：0
• ISSN：1520-5118

文摘

The antiallergic potential of Arctium lappa L. was investigated in Sprague–Dawley rats, ICR mice, and RBL-2H3 cells. Ethanol extract (90%) of A. lappa (ALE, 100 μg/mL) inhibited the degranulation rate by 52.9%, determined by the level of β-hexosaminidase. ALE suppressed passive cutaneous anaphylaxis (PCA) in rats and attenuated anaphylaxis and histamine release in mice. To identify the active compound of ALE, we subsequently fractionated and determined the level of β-hexosaminidase in all subfractions. Oleamide was identified as an active compound of ALE, which attenuated the secretion of histamine and the production of tumor necrosis factor (TNF)-α and interleukin-4 (IL-4) in cells treated with compound 48/80 or A23187/phorbol myristate acetate (PMA). Oleamide suppressed FcεRI-tyrosine kinase Lyn-mediated pathway, c-Jun N-terminal kinases (JNK/SAPK), and p38 mitogen-activated protein kinases (p38-MAPKs). These results showed that ALE and oleamide attenuated allergic reactions and should serve as a platform to search for compounds with antiallergic activity.