Antiamnesic Effect of Broccoli (Brassica oleracea var. italica) Leaves on Amyloid Beta (Aβ)1–42-Induced Learning and Memory Impairment

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• 作者：Seon Kyeong Park ; Jeong Su Ha ; Jong Min Kim ; Jin Yong Kang ; Du Sang Lee ; Tian Jiao Guo ; Uk Lee ; Dae-Ok Kim ; Ho Jin Heo
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：May 4, 2016
• 年：2016
• 卷：64
• 期：17
• 页码：3353-3361
• 全文大小：605K
• 年卷期：0
• ISSN：1520-5118

文摘

To examine the antiamnesic effects of broccoli (Brassica oleracea var. italica) leaves, we performed in vitro and in vivo tests on amyloid beta (Aβ)-induced neurotoxicity. The chloroform fraction from broccoli leaves (CBL) showed a remarkable neuronal cell-protective effect and an inhibition against acetylcholinesterase (AChE). The ameliorating effect of CBL on Aβ_1–42-induced learning and memory impairment was evaluated by Y-maze, passive avoidance, and Morris water maze tests. The results indicated improving cognitive function in the CBL group. After the behavioral tests, antioxidant effects were detected by superoxide dismutase (SOD), oxidized glutathione (GSH)/total GSH, and malondialdehyde (MDA) assays, and inhibition against AChE was also presented in the brain. Finally, oxo-dihydroxy-octadecenoic acid (oxo-DHODE) and trihydroxy-octadecenoic acid (THODE) as main compounds were identified by quadrupole time-of-flight ultraperformance liquid chromatography (Q-TOF UPLC-MS) analysis. Therefore, our studies suggest that CBL could be used as a natural resource for ameliorating Aβ_1–42-induced learning and memory impairment.