Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris

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• 作者：Chengcheng Zhao ; Xin Gao ; Xinbin Liu ; Yong Wang ; Shengli Yang ; Fengqing Wang ; Yuhong Ren
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：May 4, 2016
• 年：2016
• 卷：64
• 期：17
• 页码：3380-3385
• 全文大小：319K
• 年卷期：0
• ISSN：1520-5118

文摘

Ginsenosides from the edible and medicinal plant ginseng have demonstrated various pharmacological activities. However, producing ginsenoside efficiently remains a challenge. Engineering metabolic pathways through protein assembly in yeast is a promising way for ginsenoside production. In the biosynthetic pathway of ginsenosides, dammarenediol-II synthase and squalene epoxidase are two key enzymes that determine the production rate of the dammarane-type ginsenoside precursor dammarenediol-II. In this work, a strategy to enhance the biosynthesis of dammarenediol-II in Pichia pastoris was developed by the self-assembly of the two key enzymes via protein–protein interaction. After being modified by interacting proteins, the two enzymes were successfully co-localized, resulting in a 2.1-fold enhancement in dammarenediol-II yields.