Effects of Oleanane-Type Triterpene Saponins from the Leaves of Eleutherococcus senticosus in an Axonal Outgrowth Assay

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• 作者：Yue-Wei Ge ; Chihiro Tohda ; Shu Zhu ; Yu-Min He ; Kayo Yoshimatsu ; Katsuko Komatsu
• 刊名：Journal of Natural Products
• 出版年：2016
• 出版时间：July 22, 2016
• 年：2016
• 卷：79
• 期：7
• 页码：1834-1841
• 全文大小：383K
• 年卷期：0
• ISSN：1520-6025

文摘

An aqueous extract of Eleutherococcus senticosus leaves exerted a beneficial effect in restoring the neurite outgrowth from Aβ_25–35-induced degeneration using an axonal density assay. Subsequent bioassay-guided fractionation afforded seven new oleanane-type triterpene saponins, ezoukoginosides A–G (1–7), along with nine known analogues. The structures of 1–7 were elucidated through chemical and spectroscopic approaches, and their effects on restoring the neurite outgrowth from Aβ_25–35-induced degeneration were investigated. The results revealed that hydrophilic oleanane-type saponins substituted with a free carboxylic acid, hydroxy, or formyl group in the aglycone, especially when the oxidation occurred at C-29, not only restrained Aβ_25–35-induced degeneration but also restored axonal outgrowth significantly. Compounds 2 (−COOH at C-29) and 3 (−CH₂OH at C-29) showed the most potent bioactivity among the isolates.