Molecular MRI in the Earth’s Magnetic Field Using Continuous Hyperpolarization of a Biomolecule in Water

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• 作者：Philipp Rovedo ; Stephan Knecht ; Tim Bäumlisberger ; Anna Lena Cremer ; Simon B. Duckett ; Ryan E. Mewis ; Gary G. R. Green ; Michael Burns ; Peter J. Rayner ; Dieter Leibfritz ; Jan G. Korvink ; Jürgen Hennig ; Gerhard Pütz ; Dominik von Elverfeldt ; Jan-Bernd Hövener
• 刊名：Journal of Physical Chemistry B
• 出版年：2016
• 出版时间：June 30, 2016
• 年：2016
• 卷：120
• 期：25
• 页码：5670-5677
• 全文大小：408K
• 年卷期：0
• ISSN：1520-5207

文摘

In this work, we illustrate a method to continuously hyperpolarize a biomolecule, nicotinamide, in water using parahydrogen and signal amplification by reversible exchange (SABRE). Building on the preparation procedure described recently by Truong et al. [ <cite>J. Phys. Chem. Bcite>,class="NLM_x">ce.acs.org/2008/acs" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:space="preserve"> 2014class="NLM_x">ce.acs.org/2008/acs" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:space="preserve">, 118class="NLM_x">ce.acs.org/2008/acs" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:space="preserve">, 13882−13889], aqueous solutions of nicotinamide and an Ir-IMes catalyst were prepared for low-field NMR and MRI. The ¹H-polarization was continuously renewed and monitored by NMR experiments at 5.9 mT for more than 1000 s. The polarization achieved corresponds to that induced by a 46 T magnet (P = 1.6 × 10^–4) or an enhancement of 10⁴. The polarization persisted, although reduced, if cell culture medium (DPBS with Ca²⁺ and Mg²⁺) or human cells (HL-60) were added, but was no longer observable after the addition of human blood. Using a portable MRI unit, fast ¹H-MRI was enabled by cycling the magnetic field between 5 mT and the Earth’s field for hyperpolarization and imaging, respectively. A model describing the underlying spin physics was developed that revealed a polarization pattern depending on both contact time and magnetic field. Furthermore, the model predicts an opposite phase of the dihydrogen and substrate signal after one exchange, which is likely to result in the cancelation of some signal at low field.