Conjugated Polyelectrolyte Nanoparticles for Apoptotic Cell Imaging

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• 作者：Yu Liu ; Pan Wu ; Jianhua Jiang ; Jiatao Wu ; Yan Chen ; Ying Tan ; Chunyan Tan ; Yuyang Jiang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：August 31, 2016
• 年：2016
• 卷：8
• 期：34
• 页码：21984-21989
• 全文大小：399K
• 年卷期：0
• ISSN：1944-8252

文摘

Three anionic conjugated polyelectrolytes (CPEs) with poly(p-phenylene ethynylene thiophene) backbones were designed and synthesized, among which PPET3-CO<sub>2sub>Na showed greater molar extinction coefficient with red-shifted bands in both absorption and emission spectra compared to the well-studied PPE-CO<sub>2sub>Na polymer. PPET3-CO<sub>2sub>Na was thus chosen to construct CPE-based nanoparticles (CPNs) with cationic octaarginine (R8) peptide through electrostatic-interaction-induced self-assembly. Due to plasma membrane permeabilization and mitochondrial outer membrane permeabilization (MOMP) in early apoptotic cells, PPET3/R8 CPNs demonstrated excellent colocalization with MitoTracker Red in apoptotic cells instead of normal cells, which had potential application in cell imaging for early apoptosis recognition.