Rapid and Selective Detection of Pathogenic Bacteria in Bloodstream Infections with Aptamer-Based Recognition

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• 作者：Haijing Shen ; Jie Wang ; Haoyang Liu ; Zhihao Li ; Fenglei Jiang ; Fu-Bing Wang ; Quan Yuan
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：August 3, 2016
• 年：2016
• 卷：8
• 期：30
• 页码：19371-19378
• 全文大小：487K
• 年卷期：0
• ISSN：1944-8252

文摘

Sepsis and bacteremia are life-threatening clinical syndromes associated with significant patient morbidity and mortality. Rapid and sensitive detection of pathogenic bacteria is the key to improve patient survival rates. Herein, we have rationally constructed a simple aptamer-based capture platform to shorten the time needed for confirmation of bacterial bloodstream infection in clinical blood samples. This capture platform is made of a mesoporous TiO₂-coated magnetic nanoparticle and is modified with target aptamer. It features excellent bacterial enrichment efficiency of about 80% even at low bacterial concentrations (10–2000 CFU mL^–1). More importantly, the bacteria can be enriched within 2 h, and the time for bacterial identification is effectively shortened in comparison to the “gold standard” in clinical diagnosis of bloodstream infection. The aptamer-based capture platform may pave a way for the detection of biomarkers and find potential applications in disease diagnosis.