NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition

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• 作者：Xiaoyue Qi ; Long Chen ; Chaoqun Zhang ; Xinyuan Xu ; Yiding Zhang ; Yu Bai ; Huwei Liu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：July 27, 2016
• 年：2016
• 卷：8
• 期：29
• 页码：18675-18683
• 全文大小：478K
• 年卷期：0
• ISSN：1944-8252

文摘

A bifunctional affinity probe NiCoMnO₄ was designed and prepared with controllable morphology and size using facile methods. It was observed that the probe could be applied in His-tagged proteins purification and phosphopeptides enrichment simply through the buffer modulation. NiCoMnO₄ particles showed satisfactory cycling performance for His-tagged proteins purification and broad pH-tolerance of loading buffer for phosphopeptides affinity. Therefore, a high-throughput, cost-effective, and efficient protein/peptide purification method was developed within 10 min based on the novel bifunctional affinity probe.