Bioinspired Dual Stimuli-Responsive Membranous System with Multiple On–Off Gates

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• 作者：Bom-yi Lee ; Seung Hyun ; Gumhye Jeon ; Eun Young Kim ; Jinhwan Kim ; Won Jong Kim ; Jin Kon Kim
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：May 11, 2016
• 年：2016
• 卷：8
• 期：18
• 页码：11758-11764
• 全文大小：468K
• 年卷期：0
• ISSN：1944-8252

文摘

Stimuli-responsive polymers have been widely used for controlled release of several biomolecules. In general, a single stimulus among various stimuli, for instance, temperature, pH, or light, has been used for these polymers. Although some stimuli are applied together, one cannot control each stimulus independently at a given stimulus-responsive polymer. However, to mimic biological system like cell membrane, multiple on–off gates utilizing independent control of dual (or multiple) stimuli should be used. Here, we introduce a stimuli-responsive membrane controlled by two orthogonal stimuli. For this purpose, the top and the bottom parts of anodized aluminum oxide membrane walls are independently grafted by thermoresponsive poly(N-isopropylacrylamide) and pH-responsive poly(acrylic acid), respectively, by using surface-initiated atom transfer radical polymerization. The membrane clearly showed two independent on–off gates depending on temperature and pH. Furthermore, through light irradiation of two different wavelengths (near-infrared and ultraviolet), temperature and pH were also controlled independently and promptly. Thus, this membrane shows two independent on–off gating of the transport of a model biomolecule of fluorescein isothiocyanate-labeled bovine serum albumin. This strategy suggests the potential of independently modified membrane in layers as stimuli-responsive on–off gates for the application of artificial cell membrane.