Reversible and Dynamic Fluorescence Imaging of Cellular Redox Self-Regulation Using Fast-Responsive Near-Infrared Ge-Pyronines

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• 作者：Hailiang Nie ; Jing Jing ; Yong Tian ; Wen Yang ; Rubo Zhang ; Xiaoling Zhang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：April 13, 2016
• 年：2016
• 卷：8
• 期：14
• 页码：8991-8997
• 全文大小：447K
• 年卷期：0
• ISSN：1944-8252

文摘

Cellular self-regulation of reactive oxygen species (ROS) stress via glutathione (GSH) antioxidant repair plays a crucial role in maintaining redox balance, which affects various physiological and pathological pathways. In this work, we developed a simple yet effective strategy for reversible, dynamic, and real-time fluorescence imaging of ROS stress and GSH repair, based on novel Ge-pyronine dyes (GePs). Unlike the current O-pyronine (OP) dye, the fluorescence of GePs can be quenched in GSH reduction and then greatly restored by ROS (e.g., ClO^–, ONOO^–, and HO^•) oxidation because of their unique affinity toward thiols. The “on–off” and “off–on” fluorescence switch can complete in 10 and 20 s, respectively, and exhibit excellent reversibility in vitro and in cells. GePs also show excitation in the long wavelength from the deep-red to near-infrared (NIR) (621–662 nm) region, high fluorescence quantum yield (Φ_fl = 0.32–0.44) in aqueous media, and excellent cell permeability. Our results demonstrated that GePs can be used for real-time monitoring of the reversible and dynamic interconversion between ROS oxidation and GSH reduction in living cells. GePs might be a useful tool for investigating various redox-related physiological and pathological pathways.