Quantitative Detection of MicroRNA in One Step via Next Generation Magnetic Relaxation Switch Sensing

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• 作者：Wenjing Lu ; Yiping Chen ; Zhong Liu ; Wenbo Tang ; Qiang Feng ; Jiashu Sun ; Xingyu Jiang
• 刊名：ACS Nano
• 出版年：2016
• 出版时间：July 26, 2016
• 年：2016
• 卷：10
• 期：7
• 页码：6685-6692
• 全文大小：360K
• 年卷期：0
• ISSN：1936-086X

文摘

One-step, quantitative and rapid detection of microRNA (miRNA) in tumor cells or tissues can provide critical information for clinical diagnosis and cancer treatment. In this work, we develop a magnetic relaxation switch sensing (MRS)-based miRNA sensor using magnetic microparticle (1 μm in diameter, MM₁₀₀₀)-DNA probe-magnetic nanoparticle (30 nm in diameter, MN₃₀) conjugates (MM₁₀₀₀-DNA-MN₃₀). In the presence of target miRNA, DSN enzyme selectively cleaves the DNA tether after miRNA/DNA hybridization to release MN₃₀ and leaves the miRNA intact to lead to the declustering of more MN₃₀ than before. In contrast to conventional MRS by measuring the change of transverse relaxation time (ΔT₂) induced by the aggregation or dissociation of magnetic particles in the presence of target, we use the cleaved MN₃₀ from conjugates as the direct readout of ΔT₂, which is more sensitive and stable. This MRS-based assay allows for one-step detection of 5 fM of miR-21 in urine samples, quantification of miR-21 from 100 cancer cells, and differentiation of the expression of miR-21 in tumor and surrounding tissues. The merits of this assay, rapidity, ability for quantitation, high sensitivity, and one-step operation, ensure a promising future in diagnostic technology.