In Vivo Dynamic Monitoring of Small Molecules with Implantable Polymer-Dot Transducer

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• 作者：Kai Sun ; Ying Tang ; Qiong Li ; Shengyan Yin ; Weiping Qin ; Jiangbo Yu ; Daniel T. Chiu ; Yubin Liu ; Zhen Yuan ; Xuanjun Zhang ; Changfeng Wu
• 刊名：ACS Nano
• 出版年：2016
• 出版时间：July 26, 2016
• 年：2016
• 卷：10
• 期：7
• 页码：6769-6781
• 全文大小：885K
• 年卷期：0
• ISSN：1936-086X

文摘

Small molecules participate extensively in various life processes. However, specific and sensitive detection of small molecules in a living system is highly challenging. Here, we describe in vivo real-time dynamic monitoring of small molecules by a luminescent polymer-dot oxygen transducer. The optical transducer combined with an oxygen-consuming enzyme can sensitively detect small-molecule substrates as the enzyme-catalyzed reaction depletes its internal oxygen reservoir in the presence of small molecules. We exemplify this detection strategy by using glucose-oxidase-functionalized polymer dots, yielding high selectivity, large dynamic range, and reversible glucose detection in cell and tissue environments. The transducer–enzyme assembly after subcutaneous implantation provides a strong luminescence signal that is transdermally detectable and continuously responsive to blood glucose fluctuations for up to 30 days. In view of a large library of oxygen-consuming enzymes, this strategy is promising for in vivo detection and quantitative determination of a variety of small molecules.