Osteoblast-Targeting-Peptide Modified Nanoparticle for siRNA/microRNA Delivery

详细信息    查看全文

• 作者：Yao Sun ; Xiongzhen Ye ; Mingxiang Cai ; Xiangning Liu ; Jia Xiao ; Chenyang Zhang ; Yayu Wang ; Li Yang ; Jiafan Liu ; Shannai Li ; Chen Kang ; Bin Zhang ; Qi Zhang ; Zuolin Wang ; An Hong ; Xiaogang Wang
• 刊名：ACS Nano
• 出版年：2016
• 出版时间：June 28, 2016
• 年：2016
• 卷：10
• 期：6
• 页码：5759-5768
• 全文大小：507K
• 年卷期：0
• ISSN：1936-086X

文摘

Antiosteoporosis gene-based drug development strategies are presently focused on targeting osteoblasts to either suppress bone loss or increase bone mass. Although siRNA/microRNA-based gene therapy has enormous potential, it is severely limited by the lack of specific cell-targeting delivery systems. We report an osteoblast-targeting peptide (SDSSD) that selectively binds to osteoblasts via periostin. We developed SDSSD-modified polyurethane (PU) nanomicelles encapsulating siRNA/microRNA that delivers drugs to osteoblasts; the data showed that SDSSD–PU could selectively target not only bone-formation surfaces but also osteoblasts without overt toxicity or eliciting an immune response in vivo. We used the SDSSD–PU delivery system to deliver anti-miR-214 to osteoblasts and our results showed increased bone formation, improved bone microarchitecture, and increased bone mass in an ovariectomized osteoporosis mouse model. SDSSD–PU may be a useful osteoblast-targeting small nucleic acid delivery system that could be used as an anabolic strategy to treat osteoblast-induced bone diseases.