Intelligent Microbial Heat-Regulating Engine (IMHeRE) for Improved Thermo-Robustness and Efficiency of Bioconversion

详细信息    查看全文

• 作者：Haiyang Jia ; Xiangying Sun ; Huan Sun ; Chenyi Li ; Yunqian Wang ; Xudong Feng ; Chun Li
• 刊名：ACS Synthetic Biology
• 出版年：2016
• 出版时间：April 15, 2016
• 年：2016
• 卷：5
• 期：4
• 页码：312-320
• 全文大小：607K
• 年卷期：0
• ISSN：2161-5063

文摘

The growth and production of microorganisms in bioconversion are often hampered by heat stress. In this study, an intelligent microbial heat-regulating engine (IMHeRE) was developed and customized to improve the thermo-robustness of Escherichia coli via the integration of a thermotolerant system and a quorum-regulating system. At the cell level, the thermotolerant system composed of different heat shock proteins and RNA thermometers hierarchically expands the optimum temperature by sensing heat changes. At the community level, the quorum-regulating system dynamically programs the altruistic sacrifice of individuals to reduce metabolic heat release by sensing the temperature and cell density. Using this hierarchical, dynamical, and multilevel regulation, the IMHeRE is able to significantly improve cell growth and production. In a real application, the production of lysine was increased 5-fold at 40 °C using the IMHeRE. Our work provides new potential for the development of bioconversion by conserving energy and increasing productivity.