Highly Polymerized Procyanidins in Brown Soybean Seed Coat with a High Radical-Scavenging Activity

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• 作者：Yasuhiro Takahata ; Mayumi Ohnishi-Kameyama ; Shu Furuta ; Masakazu Takahashi ; and Ikuo Suda
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2001
• 出版时间：December 2001
• 年：2001
• 卷：49
• 期：12
• 页码：5843 - 5847
• 全文大小：56K
• 年卷期：v.49,no.12(December 2001)
• ISSN：1520-5118

文摘

1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the 70% aqueous acetone extractfrom the seed coat of the brown soybean variety, Akita-Zairai, was investigated. The activity of theseed coat of Akita-Zairai was much higher than that of three other reddish-brown varieties, butlower than that of two black varieties, and was closely dependent on the content of phenoliccompounds. In the LH20 column chromatography of Akita-Zairai, high DPPH radical-scavengingactivities were detected in the fractions eluted with MeOH and 70% aqueous acetone. Proanthocyanidins were also detected in fractions showing high radical-scavenging activities. Matrix-assistedlaser desorption/ionization-time-of-flight mass spectrometry analysis showed that the degree ofpolymerization (DP) of the procyanidins contained in the brown or black soybean seed coat was ashigh as DP30.Keywords: Soybean; Glycine max; procyanidin polymer; radical-scavenging activity; brown seedcoat; MALDI-TOFMS