A Phenylselenium-Substituted BODIPY Fluorescent Turn-off Probe for Fluorescence Imaging of Hydrogen Sulfide in Living Cells

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• 作者：Deyan Gong ; Xiangtao Zhu ; Yuejun Tian ; Shi-Chong Han ; Min Deng ; Anam Iqbal ; Weisheng Liu ; Wenwu QinHuichen Guo
• 刊名：Analytical Chemistry
• 出版年：2017
• 出版时间：February 7, 2017
• 年：2017
• 卷：89
• 期：3
• 页码：1801-1807
• 全文大小：461K
• ISSN：1520-6882

文摘

Herein a phenylselenium-substituted BODIPY (1) fluorescent turn-off sensor was developed for the purpose to achieve excellent selectivity and sensitivity for H₂S detection based on the substitution reaction of the phenylselenide group at the 3-position with H₂S. The excess addition of hydrogen sulfide promoted further substitution of the phenylselenide group at the 5-position of the probe and was accompanied by a further decrease in fluorescence emission intensity. Sensor 1 demonstrated remarkable performance with 49-fold red color fluorescence intensity decrease at longer excitation wavelength, a low detection limit (0.0025 μM), and specific fluorescent response toward H₂S over anions, biothiols, and other amino acids in neutral media. It showed no obvious cell toxicity and good membrane permeability, which was well exploited for intracellular H₂S detection and imaging through fluorescence microscopy imaging.