Near-Infrared Probe Based on Rhodamine Derivative for Highly Sensitive and Selective Lysosomal pH Tracking

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• 作者：Guangle NiuPanpan Zhang ; Weimin Liu ; Mengqi WangHongyan Zhang ; Jiasheng Wu ; Liping Zhang ; Pengfei Wang
• 刊名：Analytical Chemistry
• 出版年：2017
• 出版时间：February 7, 2017
• 年：2017
• 卷：89
• 期：3
• 页码：1922-1929
• 全文大小：609K
• ISSN：1520-6882

文摘

The development of near-infrared fluorescent probes with low pK_a, high selectivity, high photostability, and high sensitivity for lysosomal pH detection is of great importance. In the present work, we developed a novel near-infrared lysosomal pH probe (Lyso-hNR) based on a rhodamine derivative. Lyso-hNR showed fast, highly sensitive, and highly selective fluorescence response to acidic pH caused by the H⁺-induced structure changes from the nonfluorescent spirolactam form to the highly emissive open-ring form. Lyso-hNR displays a significant fluorescence enhancement at 650 nm (over 280-fold) from pH 7.0 to 4.0 with a pK_a value of 5.04. Live cell imaging data revealed that Lyso-hNR can selectively monitor lysosomal pH changes with excellent photostability and low cytotoxicity. In addition, Lyso-hNR can be successfully used in tracking lysosomal pH changes induced by chloroquine and those during apoptosis. All these features render Lyso-hNR a promising candidate to investigate lysosome-associated physiological and pathological processes.