Nano Endoscopy with Plasmon-Enhanced Fluorescence for Sensitive Sensing Inside Ultrasmall Volume Samples

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• 作者：Hang Yuan ; Jie Liu ; Yuexiang LuZhe Wang ; Guoyu Wei ; Tianhao Wu ; Gang Ye ; Jing ChenSichun Zhang ; Xinrong Zhang
• 刊名：Analytical Chemistry
• 出版年：2017
• 出版时间：January 17, 2017
• 年：2017
• 卷：89
• 期：2
• 页码：1045-1048
• 全文大小：352K
• ISSN：1520-6882

文摘

Plasmon-enhanced fluorescence (PEF) generally requires the samples settled on a metal substrate and the effective enhancement distance is less than 100 nm, which limit its application in intracellular sensing. Herein, we report a nano endoscopy with PEF effect for sensing analytes inside the extremely small volume samples. The nano endoscopy was fabricated by assembling single nanoporous gold nanowire (PGNW) on the tip of a tungsten needle. It was accurately manipulated to insert into a micro droplet, and an effective sensing was realized at micrometre scale with submicrometer resolution. By taking lysozyme as a model sensing target, a 23-fold improvement of sensitivity was obtained, comparing with that of smooth gold nanowire (SGNW). These results indicated that the nano endoscopy can realize a high spatial resolution sensing, showing its potential application in intracellular sensing.