Simple and Cost-Effective Glucose Detection Based on Carbon Nanodots Supported on Silver Nanoparticles

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• 作者：Jin-Liang Ma ; Bin-Cheng YinXin Wu ; Bang-Ce Ye
• 刊名：Analytical Chemistry
• 出版年：2017
• 出版时间：January 17, 2017
• 年：2017
• 卷：89
• 期：2
• 页码：1323-1328
• 全文大小：399K
• ISSN：1520-6882

文摘

We present a new glucose oxidase (GOx)-mediated strategy for detecting glucose based on carbon nanodots supported on silver nanoparticles (C-dots/AgNPs) as nanocomplexes. The strategy involves three processes: quenching of C-dots’ fluorescence by AgNPs, production of H₂O₂ from GOx-catalyzed oxidation of glucose, and H₂O₂-induced etching of AgNPs. In the C-dots/AgNPs complex, AgNPs act as a “nanoquencher” to decrease C-dots fluorescence by surface plasmon-enhanced energy transfer (SPEET) from C-dots (donor) to AgNPs (acceptor). The H₂O₂ formed by GOx-catalyzed oxidation of glucose etches the AgNPs to silver ions, thus freeing the C-dots from the AgNPs surfaces and restoring the C-dots’ fluorescence. Therefore, the increase in fluorescence depends directly on the concentration of H₂O₂, which, in turn, depends on the concentration of glucose. The strategy allows the quantitative analysis of glucose with a detection limit of 1.39 μM. The method based on C-dots/AgNPs offers the following advantages: simplicity of design and facile preparation of nanomaterials, as well as low experimental cost, because chemical modification and separation procedures are not needed.