Ultrasensitive Signal-On Detection of Nucleic Acids with Surface-Enhanced Raman Scattering and Exonuclease III-Assisted Probe Amplification

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• 作者：Yingying Li ; Qingcheng Zhao ; Yandong Wang ; Tiantian Man ; Lu Zhou ; Xu Fang ; Hao Pei ; Lifeng Chi ; Jian Liu
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：December 6, 2016
• 年：2016
• 卷：88
• 期：23
• 页码：11684-11690
• 全文大小：471K
• ISSN：1520-6882

文摘

The methodological development of nucleic acids detection is a rapidly growing research field. Here, we report a powerful method to detect nucleic acids by an integration of surface-enhanced Raman scattering and exonuclease III-assisted probe amplification. With a unique signal-on strategy, we have demonstrated that the target DNA of MnSOD gene in concentrations as low as 1 aM can reproducibly be detected, which offers a detection limit several orders of magnitude better than the previous reports in the literature. The new biosensor exhibits an excellent specificity in differentiating DNA sequences with a single-base mismatch. As a robust, flexible, and ultrasensitive approach, it promises important applications in clinical diagnostics and DNA identification where only a very limited amount of the biological sample is available.