Lauric Acid Stimulates Mammary Gland Development of Pubertal Mice through Activation of GPR84 and PI3K/Akt Signaling Pathway

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• 作者：Yingying Meng ; Jing Zhang ; Fenglin Zhang ; Wei Ai ; Xiaotong Zhu ; Gang Shu ; Lina Wang ; Ping Gao ; Qianyun Xi ; Yongliang Zhang ; Xingwei Liang ; Qingyan Jiang ; Songbo Wang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2017
• 出版时间：January 11, 2017
• 年：2017
• 卷：65
• 期：1
• 页码：95-103
• 全文大小：623K
• ISSN：1520-5118

文摘

It has been demonstrated that dietary fat affects pubertal mammary gland development. However, the role of lauric acid (LA) in this process remains unclear. Thus, this study aimed to investigate the effects of LA on mammary gland development in pubertal mice and to explore the underlying mechanism. In vitro, 100 μM LA significantly promoted proliferation of mouse mammary epithelial cell line HC11 by regulating expression of proliferative markers (cyclin D1/3, p21, PCNA). Meanwhile, LA activated the G protein-coupled receptor 84 (GPR84) and PI3K/Akt signaling pathway. In agreement, dietary 1% LA enhanced mammary duct development, increased the expression of GPR84 and cyclin D1, and activated PI3K/Akt in mammary gland of pubertal mice. Furthermore, knockdown of GPR84 or inhibition of PI3K/Akt totally abolished the promotion of HC11 proliferation induced by LA. These results showed that LA stimulated mammary gland development of pubertal mice through activation of GPR84 and PI3K/Akt signaling pathway.