Fluorescent pH-Sensing Probe Based on Biorefinery Wood Lignosulfonate and Its Application in Human Cancer Cell Bioimaging

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• 作者：Yuyuan Xue ; Wanshan Liang ; Yuan Li ; Ying Wu ; Xinwen PengXueqing Qiu ; Jinbin Liu ; Runcang Sun
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：December 28, 2016
• 年：2016
• 卷：64
• 期：51
• 页码：9592-9600
• 全文大小：542K
• ISSN：1520-5118

文摘

A water-soluble, ratiometric fluorescent pH probe, L-SRhB, was synthesized via grafting spirolactam Rhodamine B (SRhB) to lignosulfonate (LS). As the ring-opening product of L-SRhB, FL-SRhB was also prepared. The pH-response experiment indicated that L-SRhB showed a rapid response to pH changes from 4.60 to 6.20 with a pK_a of 5.35, which indicated that L-SRhB has the potential for pH detection of acidic organelle. In addition, the two probes were internalized successfully by living cells through the endocytosis pathway and could distinguish normal cells from cancer cells by different cell staining rates. In addition, L-SRhB showed obvious cytotoxicity to cancer cells, whereas it was nontoxic to normal cells in the same condition. L-SRhB might have potential in cancer therapy. L-SRhB might be a promising ratiometric fluorescent pH sensor and bioimaging dye for the recognition of cancer cells. The results also provided a new perspective to the high-value utilization of lignin.