Functional Properties of Novel Epigallocatechin Gallate Glucosides Synthesized by Using Dextransucrase from Leuconostoc mesenteroides B-1299CB4

详细信息    查看全文

• 作者：Jiyoun Kim ; Thi Thanh Hanh Nguyen ; Nahyun M. Kim ; Young-Hwan Moon ; Jung-Min Ha ; Namhyeon Park ; Dong-Gu Lee ; Kyeong-Hwan Hwang ; Jun-Seong Park ; Doman Kim
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：December 7, 2016
• 年：2016
• 卷：64
• 期：48
• 页码：9203-9213
• 全文大小：594K
• ISSN：1520-5118

文摘

Epigallocatechin gallate (EGCG) is the most abundant catechin found in the leaves of green tea, Camellia sinensis. In this study, novel epigallocatechin gallate-glucocides (EGCG-Gs) were synthesized by using dextransucrase from Leuconostoc mesenteroides B-1299CB4. Response surface methodology was adopted to optimize the conversion of EGCG to EGCG-Gs, resulting in a 91.43% conversion rate of EGCG. Each EGCG-G was purified using a C₁₈ column. Of nine EGCG-Gs identified by nuclear magnetic resonance analysis, five EGCG-Gs (2 and 4–7) were novel compounds with yields of 2.2–22.6%. The water solubility of the five novel compounds ranged from 229.7 to 1878.5 mM. The 5′-OH group of EGCG-Gs expressed higher antioxidant activities than the 4′-OH group of EGCG-Gs. Furthermore, glucosylation at 7-OH group of EGCG-Gs was found to be responsible for maintaining tyrosinase inhibitory activity and increasing browning-resistant activities.