Real-Time Screening of Biocatalysts in Live Bacterial Colonies
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- 作者：Cunyu YanFabio Parmeggiani ; Emrys A. JonesEmmanuelle Claude ; Shaneela A. Hussain ; Nicholas J. Turner ; Sabine L. Flitsch ; Perdita E. Barran
- 刊名：Journal of the American Chemical Society
- 出版年：2017
- 出版时间：February 1, 2017
- 年：2017
- 卷：139
- 期：4
- 页码：1408-1411
- 全文大小：408K
- ISSN：1520-5126
文摘
Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection methods. Herein we report a label-free screening platform for biotransformations in live colonies using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DiBT-IMMS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.