Selective Visualization of the Endogenous Peroxynitrite in an Inflamed Mouse Model by a Mitochondria-Targetable Two-Photon Ratiometric Fluorescent Probe

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• 作者：Dan Cheng ; Yue Pan ; Lu Wang ; Zebing ZengLin Yuan ; Xiaobing ZhangYoung-Tae Chang
• 刊名：Journal of the American Chemical Society
• 出版年：2017
• 出版时间：January 11, 2017
• 年：2017
• 卷：139
• 期：1
• 页码：285-292
• 全文大小：554K
• ISSN：1520-5126

文摘

Peroxynitrite (ONOO^–) is a kind of reactive oxygen species (ROS) with super activity of oxidization and nitration, and overproduction of ONOO^– is associated with pathogenesis of many diseases. Thus, accurate detection of ONOO^– with high sensitivity and selectivity is imperative for elucidating its functions in health or disease states. Herein we for the first time present a new two-photon ratiometric fluorescent ONOO^– probe (MITO-CC) based on FRET mechanism by combining rational design strategy and dye-screening approach. MITO-CC, with fast response rate (within 20 s), excellent sensitivity (detection limit = 11.30 nM) and outstanding selectivity toward ONOO^–, was successfully applied to ratiometric detection of endogenous ONOO^– produced by HepG2/RAW264.7 cells and further employed for imaging oxidative stress in an inflamed mouse model. Therefore, probe MITO-CC could be a potential biological tool to explore the roles of ONOO^– under different physiological and pathological settings.