Identification of Serratane Synthase Gene from the Fern Lycopodium clavatum

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• 作者：Yusuke Saga ; Takeshi Araki ; Hiroshi Araya ; Kazuki Saito ; Mami Yamazaki ; Hideyuki Suzuki ; Tetsuo Kushiro
• 刊名：Organic Letters
• 出版年：2017
• 出版时间：February 3, 2017
• 年：2017
• 卷：19
• 期：3
• 页码：496-499
• 全文大小：306K
• ISSN：1523-7052

文摘

Ferns are known to produce onoceroids including onoceranes and serratanes having unusual structures among triterpenes. From the fern Lycopodium clavatum, a novel onoceroid synthase gene was cloned that showed high sequence identity with a previously identified α-onocerin synthase. Functional analysis by coexpression with pre-α-onocerin synthase in yeast led to the production of tohogenol and serratenediol. The result suggested that serratanes are directly biosynthesized from pre-α-onocerin and not from α-onocerin as previously assumed.