pH-Dependent Fluorescent Probe That Can Be Tuned for Cysteine or Homocysteine

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• 作者：Yongkang Yue ; Fangjun Huo ; Xiaoqi Li ; Ying Wen ; Tao Yi ; James Salamanca ; Jorge O. Escobedo ; Robert M. Strongin ; Caixia Yin
• 刊名：Organic Letters
• 出版年：2017
• 出版时间：January 6, 2017
• 年：2017
• 卷：19
• 期：1
• 页码：82-85
• 全文大小：390K
• ISSN：1523-7052

文摘

The very close structural similarities between cysteine and homocysteine present a great challenge to achieve their selective detection using regular fluorescent probes, limiting the biological and pathological studies of these two amino thiols. A coumarin-based fluorescent probe was designed featuring pH-promoted distinct turn-on followed by ratiometric fluorescence responses for Cys and turn-on fluorescence response for Hcy through two different reaction paths. These specific responses demonstrate the activity differences between Cys and Hcy qualitatively for the first time. The probe could also be used for Cys and Hcy imaging in living cells.