Upconversion Nanocrystals Mediated Lateral-Flow Nanoplatform for in Vitro Detection

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• 作者：Zhiqin Liang ; Xiaochen Wang ; Wei Zhu ; Pingping Zhang ; Yongxin Yang ; Chongyun Sun ; Junjie Zhang ; Xinrui Wang ; Zheng Xu ; Yong Zhao ; Ruifu Yang ; Suling ZhaoLei Zhou
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2017
• 出版时间：February 1, 2017
• 年：2017
• 卷：9
• 期：4
• 页码：3497-3504
• 全文大小：529K
• ISSN：1944-8252

文摘

Upconversion phosphors (UCPs) that are free from interference from biological sample autofluorescence have attracted attention for in vivo and in vitro bioapplications. However, UCPs need to be water-dispersible, nanosized, and highly luminous to realize broad applications. Therefore, the aim of this research is to develop UCPs that meet these comprehensive criteria for in vitro diagnosis. To combine nano size with high luminous intensity, β-NaYF₄:Yb³⁺,Er³⁺ upconversion nanocrystals (UCNPs) codoped with Li⁺ and K⁺ are prepared that display high upconversion intensities as well as small size. The strongest green and red emissions of the Na_0.9Li_0.07K_0.03YF₄:Yb³⁺,Er³⁺ nanocrystals are increased by 7 and 10 times, respectively, compared with those of the undoped NaYF₄:Yb³⁺,Er³⁺ nanocrystals. A mild sol–gel surface modification method is used to produce water-phase dispersions and allow covalent biomolecule conjugation. The bioactivated UCNPs are used as a bioreporter and integrated with a classical lateral flow assay to establish an assay to accomplish simultaneous dual-target detection of Yersinia pestis and Burkholderia pseudomallei. The assay achieves a sensitivity of 10³ CFU/test without cross-interference between two targets. The research provides a way to produce UCNPs with comprehensive properties for use as excellent optical reporters in in vivo and in vitro bioapplications.