Ratiometric Fluorescent Silicon Quantum Dots–Ce6 Complex Probe for the Live Cell Imaging of Highly Reactive Oxygen Species

详细信息    查看全文

• 作者：Qianqian Zhao ; Ren Zhang ; Daixin Ye ; Song Zhang ; Hui ChenJilie Kong
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2017
• 出版时间：January 25, 2017
• 年：2017
• 卷：9
• 期：3
• 页码：2052-2058
• 全文大小：440K
• ISSN：1944-8252

文摘

The monitoring of reactive oxygen species (ROS) in living cells remains challenging because of the complexity, short half-life, and autofluorescence of biological samples. In this work, we designed a ratiometric fluorescent probe for the detection and imaging of ROS, which was constructed from silicon quantum dots (Si QDs) with chlorin e6 (Ce6) through electrostatic attraction and showed well-resolved dual fluorescence emission signals (490 and 660 nm). Sensitive and selective biosensing of hydroxyl radical (^•OH) was demonstrated on the basis of fluorescence quenching of the Si QDs and Ce6 as an internal reference to avoid environmental interference, with a detection limit of ∼0.97 μM. The endogenous release of ^•OH was also monitored and imaged in living cells.