Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry

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• 作者：Congcong Lu ; Shanshan Tian ; Guijin Zhai ; Zuofei Yuan ; Yijun Li ; Xiwen He ; Yukui Zhang ; Kai Zhang
• 刊名：ACS Chemical Biology
• 出版年：2017
• 出版时间：January 20, 2017
• 年：2017
• 卷：12
• 期：1
• 页码：57-62
• 全文大小：320K
• ISSN：1554-8937

文摘

Histone proteins, which could interact with DNA, play important roles in the regulation of chromatin structures, transcription, and other DNA-based biological processes. Here, we developed a novel aptamer-based probe for the analysis of histone H4-aptamer interfaces. This probe contains a DNA sequence for specific recognition of histone H4, a biotin tag for affinity enrichment, an aryl azide photoactive group for cross-linking and a cleavable disulfide group to dissociate aptamer from labeled histones. We successfully achieved specific enrichment of histone H4 and further developed a new analysis strategy for histone-aptamer interaction by photo cross-linking mass spectrometry. The binding area of histone H4 to aptamer was investigated and discussed for the first time. This strategy exhibits great potential and might further contribute to the understanding of histone–DNA interaction patterns.