Effect of Ethanol Organosolv Lignin from Bamboo on Enzymatic Hydrolysis of Avicel

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• 作者：Kai Wu ; Zhengjun Shi ; Haiyan Yang ; Zhengdiao Liao ; Jing Yang
• 刊名：ACS Sustainable Chemistry & Engineering
• 出版年：2017
• 出版时间：February 6, 2017
• 年：2017
• 卷：5
• 期：2
• 页码：1721-1729
• 全文大小：474K
• ISSN：2168-0485

文摘

The interactions between lignin and cellulase play a key role in the effective enzyme saccharification of lignocellulose. This work investigated the enhancing effect of ethanol organosolv lignins (EOL) from bamboo on enzyme hydrolysis of the pure cellulose. The addition of EOL-P. amarus and EOL-D. sinicus (8 g/L) remarkably increased the 72 h glucose yields of Avicel, from 51.3% to 61.1%, and from 51.3% to 59.4%, respectively, and made much more cellulase available for cellulose. Langmuir adsorption isotherms showed that ethanol organosolv lignins from bamboo had a lower binding ability to the cellulase enzymes than milled wood lignin (MWL), which resulted in less nonproductive binding. Also, FTIR and ¹³C and 2D HSQC NMR spectra showed that lignin degradation occurred by cleavaging β-O-4 linkages in the ethanol organosolv process; EOL-D. sinicus showed a less condensed structure of isolated lignins at the C_β position than MWL-D. sinicus. In addition, carboxylic groups, ferulic acid, and p-coumarate acid units exposed in ethanol organosolv pretreatment led to an increase in hydrophilicity and negative charges, and could be responsible for the promoting effect of enzymatic hydrolysis.