Structural Variation of Lignin and Lignin–Carbohydrate Complex in Eucalyptus grandis × E. urophylla during Its Growth Process

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• 作者：Bao-Cheng Zhao ; Bo-Yang Chen ; Sheng Yang ; Tong-Qi Yuan ; Adam Charlton ; Run-Cang Sun
• 刊名：ACS Sustainable Chemistry & Engineering
• 出版年：2017
• 出版时间：January 3, 2017
• 年：2017
• 卷：5
• 期：1
• 页码：1113-1122
• 全文大小：593K
• ISSN：2168-0485

文摘

Milled wood lignin (MWL), lignin–carbohydrate complex-rich fraction (LCC-AcOH), celluloytic enzyme lignin (CEL), and enzymatic hydrolysis residue (EHR) were sequentially isolated from Eucalyptus grandis × E. urophylla under mild conditions, and the variations of lignin–carbohydrate complex (LCC) linkages and lignin structures during the eucalyptus growth were investigated. The 2D HSQC NMR analysis showed that β-O-4′ and β–β′ were the main linkages in lignin, while other substructures were present in much lower amounts. The amounts of β-O-4′ in the MWL and LCC-AcOH fractions showed an increased tendency and those in the CEL and EHR fractions had no obvious variation with the eucalyptus growth. The S/G ratios of the MWL, LCC-AcOH, and CEL fractions increased first and then decreased, whereas those of the EHR fractions decreased with the tree age. The amount of phenyl glycoside (PhGlc) in the LCC-AcOH fractions varied consistent with the S/G ratio. The variation of the amount of benzyl ether (BE) in the MWL fractions was parallel to the S/G ratio, while that in the CEL fractions was contrary to it. These findings will provide some evidence for the structural variation of lignin and LCC in Eucalypt during its growth process.