Slippery Liquid-Infused Porous Surfaces that Prevent Bacterial Surface Fouling and Inhibit Virulence Phenotypes in Surrounding Planktonic Cells

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• 作者：Michael J. Kratochvil ; Michael A. Welsh ; Uttam Manna ; Benjamín J. Ortiz ; Helen E. Blackwell ; David M. Lynn
• 刊名：ACS Infectious Diseases
• 出版年：2016
• 出版时间：July 8, 2016
• 年：2016
• 卷：2
• 期：7
• 页码：509-517
• 全文大小：501K
• ISSN：2373-8227

文摘

Surfaces that can both prevent bacterial biofouling and inhibit the expression of virulence phenotypes in surrounding planktonic bacteria are of interest in a broad range of contexts. Here, we report new slippery-liquid infused porous surfaces (SLIPS) that resist bacterial colonization (owing to inherent “slippery” surface character) and also attenuate virulence phenotypes in non-adherent cells by gradually releasing small-molecule quorum sensing inhibitors (QSIs). QSIs active against Pseudomonas aeruginosa can be loaded into SLIPS without loss of their slippery and antifouling properties, and imbedded agents can be released into surrounding media over hours to days depending on the structures of the loaded agent. This controlled-release approach is useful for inhibiting virulence factor production and can also inhibit bacterial biofilm formation on nearby, non-SLIPS-coated surfaces. Finally, we demonstrate that this approach is compatible with the simultaneous release of more than one type of QSI, enabling greater control over virulence and suggesting new opportunities to tune the antifouling properties of these slippery surfaces.