Quantification of Uronic Acids in Tea Polysaccharide Conjugates and Their Antioxidant Properties

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• 作者：Haixia Chen ; Min Zhang ; and Bijun Xie
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：June 2, 2004
• 年：2004
• 卷：52
• 期：11
• 页码：3333 - 3336
• 全文大小：51K
• 年卷期：v.52,no.11(June 2, 2004)
• ISSN：1520-5118

文摘

A technique of high-performance liquid chromatography (HPLC) was described for the measurementof total uronic acids in tea polysaccharide conjugates. This method was applied to polysaccharideconjugate extracts obtained from green tea after most of the components that produce interferencewere removed. The preliminary extraction process was according to the procedure of isolation ofpolysaccharide conjugates. The uronic acid content of different polysaccharide conjugate fractionswas quantified by HPLC on a Sugar-Pak I column with a 1.0 × 10^-4 mol L^-1 calcium disodiumethylenediaminetetraacetic acid solution as the mobile phase and refractive index detection. Thevalidation study showed high recoveries (>97.0%) and low coefficients of variance (<3.0%). Theminimum detectable limit concentration of uronic acid was 10 g mL^-1. The analysis of a standardrange of galacturonic acid concentrations (100-4000 g mL^-1) yielded linear results. The use of themethod on different polysaccharide conjugate fraction samples confirmed its effectiveness. With thehigh content of uronic acids in polysaccharide conjugates, the stronger reactive oxygen speciesscavenging activities were found.Keywords: HPLC; uronic acid; tea polysaccharide conjugates; antioxidant properties