Anti-inflammatory Inhibition of Endothelial Cell Adhesion Molecule Expression by Flavone Derivatives

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• 作者：Hyang-Mi Kwon ; Yean-Jung Choi ; Yu-Jin Jeong ; Sang-Wook Kang ; Il-Jun Kang ; Soon Sung Lim ; and Young-Hee Kang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：June 29, 2005
• 年：2005
• 卷：53
• 期：13
• 页码：5150 - 5157
• 全文大小：387K
• 年卷期：v.53,no.13(June 29, 2005)
• ISSN：1520-5118

文摘

Endothelial expression of cell adhesion molecules (CAM) including VCAM-1, E-selectin, and PECAM-1plays a leading role in atherosclerosis. Phenolic flavones have been shown to have an anti-inflammatory property. This study examines whether 3',4'-dimethoxy-7-hydroxyflavone (methoxyflavone) and 2',3',7-trihydroxyflavone (hydroxyflavone) inhibited monocyte adhesion to TNF--activatedendothelium via reduction of CAM expression in human umbilical vein endothelial cells (HUVEC). Instimulated HUVEC the expression of VCAM-1 and E-selectin was enhanced with increasing mRNAlevels. Methoxyflavone markedly interfered with the THP-1 monocyte adhesion to TNF--stimulatedHUVEC. At concentrations of 25 M, methoxyflavone blocked the induction of VCAM-1 but notthat of E-selectin on the activated HUVEC. Immunocytochemical staining showed that methoxyflavonemodestly inhibited PECAM-1 expression induced by TNF-. In contrast, hydroxyflavone minimallyinhibited TNF--stimulated E-selectin expression without affecting VCAM-1 level. The inhibitory effectof methoxyflavone on THP-1 adhesion to HUVEC appears to be greater than that of hydroxyflavone,most likely due to a greater inhibition of CAM expression. Thus, some flavone derivatives containingmethoxy groups may have therapeutic potential attenuating inflammatory response-related atherosclerosis.Keywords: Flavone derivatives; tumor necrosis-; cell adhesion molecules; endothelium