Disposable Biosensor Test for Organophosphate and Carbamate Insecticides in Milk

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• 作者：Yaodong Zhang ; Susanne B. Muench ; Holger Schulze ; Roland Perz ; Bolun Yang ; Rolf D. Schmid ; and Till T. Bachmann
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：June 29, 2005
• 年：2005
• 卷：53
• 期：13
• 页码：5110 - 5115
• 全文大小：80K
• 年卷期：v.53,no.13(June 29, 2005)
• ISSN：1520-5118

文摘

A highly sensitive and rapid biosensor test based on disposable screen-printed thick-film electrodeswas developed, which is suitable for monitoring organophosphate and carbamate residues in foodsof animal origin with increased fat contents such as milk. The wild-type enzyme was combined withthree engineered variants of Nippostrongylus brasiliensis acetylcholinesterase (NbAChE), to obtainenhanced sensitivity. The sample pretreatment could be reduced to a minimum. There was noextraction or fat removal necessary. With the biosensor test paraoxon concentrations down to 1 g/Lcould be detected in milk. The detection limit for carbaryl was 20 g/L. Recovery rates for paraoxonand carbaryl in milk samples lay between 89 and 107%. Ten milk samples from local markets weretested both with the biosensor test and with standard chromatographic multiresidue methods. Twomilk samples caused AChE inhibition rates of >50%. Accordingly, 4 g/L tebufenpyrad, 4 g/Ltetraconazole, and 2 g/L bifenthrin were detected in one of these milk samples. The other milksample contained 2 g/L tebufenpyrad.Keywords: Acetylcholinesterase biosensor; organophosphate; carbamate; milk analysis