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• 作者：Jae Sung Lee ; Sang Heon Kim ; Young Jin Kim ; Toshihiro Akaike ; and Sung Chul Kim
• 刊名：Biomacromolecules
• 出版年：2005
• 出版时间：July 2005
• 年：2005
• 卷：6
• 期：4
• 页码：1906 - 1911
• 全文大小：607K
• 年卷期：v.6,no.4(July 2005)
• ISSN：1526-4602

文摘

A surface of poly(L-lactic acid) (PLLA) was modified by coating with poly[N-p-vinylbenzyl-4-O--D-galactopyranosyl-D-glucoamide] (PVLA), which was employed to improve the hepatocyte adhesion owingto its amphiphilic property and the presence of a hepatocyte recognition motif. We characterized the surfaceproperties through water contact angle, electron spectroscopy for chemical analysis (ESCA), and scanningprobe microscopy (SPM). The effect of PVLA coating on the efficiency of hepatocyte adhesion was evaluatedby protein assay and optical microscopy. The surface morphology was under the influence of the concentrationof PVLA coating solution and it played a critical role in hepatocyte adhesion. It was confirmed that galactosemoieties in PVLA, which can bind to the asialoglycoprotein receptor (ASGPR) on hepatocytes, have amore dominant effect on hepatocyte adhesion than enhanced hydrophilicity. We suggest that the PVLA-PLLA system will be a useful method to improve hepatocyte cell seeding and adhesion onto scaffold matrices.