Chemical and Biological Assessment of a Chinese Herbal Decoction Containing Radix Astragali and Radix Angelicae Sinensis: Determination of Drug Ratio in Having Optimized Properties

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• 作者：Tina T. X. Dong ; Kui J. Zhao ; Qiu T. Gao ; Zhao N. Ji ; Ting T. Zhu ; Jun Li ; Ran Duan ; Anna W. H. Cheung ; and Karl W. K. Tsim
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：April 5, 2006
• 年：2006
• 卷：54
• 期：7
• 页码：2767 - 2774
• 全文大小：154K
• 年卷期：v.54,no.7(April 5, 2006)
• ISSN：1520-5118

文摘

Danggui Buxue Tang (DBT), a Chinese medicinal decoction that is commonly used as a dietarysupplement in treating woman with menopausal irregularity, contains two herbs: Radix Astragali(Huangqi) and Radix Angelicae Sinensis (Danggui). The ratio of Radix Astragali and Radix AngelicaeSinensis used in DBT should be 5:1 as described in China in 1247 A.D.; however, the rationale ofthis formula has not been given. Here, the chemical and biological properties of DBT, prepared fromdifferent ratios of the drugs, were determined. Significantly, higher amounts of Radix Astragali-derivedastragaloside IV, calycosin, and formononetin and Radix Angelicae Sinensis-derived ferulic acid werefound in DBT with Radix Astragali and Radix Angelicae Sinensis in a 5:1 ratio. By using the biologicaleffects of DBT in stimulating osteoblast proliferation, estrogen promoter activation, and anti-plateletaggregation activity, the drug ratio of 5:1 produced the best effects. In addition, the use of ethanol-treated Radix Angelicae Sinensis enhanced the efficacy of DBT, and the treatment further increasedthe solubilities of chemical constituents. By analyzing the correlation of chemical and biological results,several chemicals showed positive correlation with DBT-induced bioactivities. The current resultssupport the ancient formulation of DBT, and the identified chemicals could serve as markers for qualitycontrol of DBT.Keywords: Angelica sinensis; umbelliferae; Astragalus membranaceus var. mongholicus; leguminosae;TCM; analytic method