Purification of Subunit B of Shiga Toxin Using a Synthetic Trisaccharide-Based Affinity Matrix

详细信息    查看全文

• 作者：Vince Pozsgay ; Loc Trinh ; Joseph Shiloach ; John B. Robbins ; Arthur Donohue-Rolfe ; and Stephen B. Calderwood
• 刊名：Bioconjugate Chemistry
• 出版年：1996
• 出版时间：January 1996
• 年：1996
• 卷：7
• 期：1
• 页码：45 - 55
• 全文大小：553K
• 年卷期：v.7,no.1(January 1996)
• ISSN：1520-4812

文摘

The blood group P₁ antigenic trisaccharide (3),which is the receptor-binding ligand of Shiga-like toxins,is synthesized in a spacer-equipped form (32) from2-(trimethylsilyl)ethyl glucoside 5 andthe1-thiogalactoside building blocks 10 and 22 in astereocontrolled, stepwise fashion. Covalentattachment of 32 to hydrazine group-containing agarose gelby reductive amination provided the P₁trisaccharide-containing affinity sorbent which was used forpreparative scale isolation of subunit Bof Shiga toxin.