Role of Complement 3 in TNF-α-Induced Mesenchymal Transition of Renal Tubular Epithelial Cells In Vitro

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• 作者：Jianxin Wan (1)
  Xueli Zhou (1)
  Jiong Cui (1)
  Zhenhuan Zou (1)
  Yanfang Xu (1)
  Danyu You (1)
  
• 关键词：Renal tubular epithelial cells ; Epithelial mesenchymal transition ; Complement 3 ; E ; cadherin ; α ; SMA
• 刊名：Molecular Biotechnology
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：54
• 期：1
• 页码：92-100
• 全文大小：739KB
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• 作者单位：Jianxin Wan (1)
  Xueli Zhou (1)
  Jiong Cui (1)
  Zhenhuan Zou (1)
  Yanfang Xu (1)
  Danyu You (1)
  
  1. Department of Nephrology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China
  

文摘

Injured renal tubular epithelial cells (RTECs) have been recently thought to directly contribute to the accumulation of myofibroblasts in renal tubulointerstitial fibrosis through a process of epithelial to mesenchymal transition (EMT). However, the factors inducing RTECs to undergo EMT and the underlying mechanisms need to be further elucidated. This study aimed to determine the EMT-inducing activity of proinflammatory cytokine TNF-α and the role for complement 3 (C3) in this activity in an in vitro model of human RTECs (HK-2 cells). Wild type HK-2 cells were treated with TNF-α, IFN-γ or C3a; C3 siRNA- or control siRNA-carrying HK-2 cells were treated with TNF-α. Changes in the cell morphology and phenotype were assessed by microscopy, RT-PCR, western blotting, and immunostaining. TNF-α effectively induced HK-2 cells to express C3 and to transform into morphologically myofibroblast-like cells that lost E-cadherin (a classical epithelial cell marker) expression but acquired alpha-smooth muscle actin (α-SMA, a classical myofibroblast differentiation marker) expression. C3 siRNA robustly attenuated all the morphologic and phenotypic changes induced by TNF-α but the control siRNA showed no effect. Our preliminary observations suggest that TNF-α may induce EMT in RTECs through inducing C3 expression.