Localization and Behaviour of Actin Microfilaments in Laticiferous Cells of Hevea brasiliensis in Latex Exploitation

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• 作者：Zhengquan Gao (1)
  Chunxiao Meng (1)
  Naihao Ye (2)
  
• 关键词：Hevea brasiliensis ; rubber tree ; laticifer ; actin microfilaments ; latex exploitation ; wound ; protein network
• 刊名：Journal of Plant Biochemistry and Biotechnology
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：17
• 期：2
• 页码：189-192
• 全文大小：125KB
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• 作者单位：Zhengquan Gao (1)
  Chunxiao Meng (1)
  Naihao Ye (2)
  
  1. School of Life Sciences, Shandong University of Technology, 255049, Zibo, China
  2. Yellow Sea Fishery Research Institute, 106 # Nanjing Road, 266071, Qingdao, China
  

文摘

Actin microfilaments of laticiferous cells and bark wounds in Hevea brasiliensis were studied using TRITC-phalloidin fluorescent microscopy. Actin in latex from mature rubber trees was also investigated using SDS-PAGE and western-boltting. TRITC-fluorescent substance plugged the end of laticifers when latex flow stopped. Actin was detected only in C serum among the four latex fractions. Higher actin content was found in the latex collected at the beginning of tapping than in that collected just before latex flow stopped. Lower actin content was detected in the latex from rubber trees with more intensive exploitation. The present study indicated that actin microfilaments might play an important role in regulation of latex flow and plugging of the laticifers wounds.