Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum

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• 作者：Tao Zhang (14505) (24505)
  Ying Zhuo (14505)
  XiaoPeng Jia (14505) (24505)
  JinTao Liu (14505) (24505)
  Hong Gao (14505)
  FuHang Song (14505)
  Mei Liu (14505)
  LiXin Zhang (14505)
  
• 关键词：beauvericin ; Fusarium proliferatum ; biosynthesis gene cluster ; synthetic biology
• 刊名：Science China Life Sciences
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：56
• 期：7
• 页码：628-637
• 全文大小：740KB
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• 作者单位：Tao Zhang (14505) (24505)
  Ying Zhuo (14505)
  XiaoPeng Jia (14505) (24505)
  JinTao Liu (14505) (24505)
  Hong Gao (14505)
  FuHang Song (14505)
  Mei Liu (14505)
  LiXin Zhang (14505)
  
  14505. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
  24505. University of Chinese Academy of Sciences, Beijing, 100049, China
  

文摘

Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches.