Bioinformatics analysis of two-component regulatory systems in Staphylococcus epidermidis

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• 作者：Qin Zhiqiang (1)
  Zhong Yang (2) (3)
  Zhang Jian (4)
  He Youyu (5)
  Wu Yang (1)
  Jiang Juan (1)
  Chen Jiemin (1)
  Luo Xiaomin (4)
  Qu Di (1)
  
• 关键词：Staphylococcus epidermidis ; two ; component regulatory system ; histidine kinase ; response regulator ; bioinformatics ; drug target
• 刊名：Chinese Science Bulletin
• 出版年：2004
• 出版时间：June 2004
• 年：2004
• 卷：49
• 期：12
• 页码：1267-1271
• 全文大小：275KB
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• 作者单位：Qin Zhiqiang (1)
  Zhong Yang (2) (3)
  Zhang Jian (4)
  He Youyu (5)
  Wu Yang (1)
  Jiang Juan (1)
  Chen Jiemin (1)
  Luo Xiaomin (4)
  Qu Di (1)
  
  1. Ministry of Education and Health Key Laboratory for Medical Molecular Virology, Medical School of Fudan University, 200032, Shanghai, China
  2. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, 200433, Shanghai, China
  3. Shanghai Center for Bioinformation Technology, 201203, Shanghai, China
  4. Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 201203, Shanghai, China
  5. Bioinformatics Center, Shanghai Institutes for Biological Studies, Chinese Academy of Sciences, 200031, Shanghai, China
  
• ISSN：1861-9541

文摘

Sixteen pairs of two-component regulatory systems are identified in the genome of Staphylococcus epidermidis ATCC12228 strain, which is newly sequenced by our laboratory for Medical Molecular Virology and Chinese National Human Genome Center at Shanghai, by using bioinformatics analysis. Comparative analysis of the twocomponent regulatory systems in S. epidermidis and that of S. aureus and Bacillus subtilis shows that these systems may regulate some important biological functions, e.g. growth, biofilm formation, and expression of virulence factors in S. epidermidis. Two conserved domains, i.e. HATPase c and REC domains, are found in all 16 pairs of two-component proteins. Homologous modelling analysis indicates that there are 4 similar HATPase c domain structures of histidine kinases and 13 similar REC domain structures of response regulators, and there is one AMP-PNP binding pocket in the HATPase c domain and three active aspartate residues in the REC domain. Preliminary experiment reveals that the bioinformatics analysis of the conserved domain structures in the two-component regulatory systems in S. epidermidis may provide useful information for discovery of potential drug target.