The inhibiting effect of 1·4 recombinant P chromosome of wheat-Agropyron cristatum addition line on the Ph gene

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• 作者：GuoHui Yang (1)
  XinMing Yang (1)
  RuiHui Wang (1)
  AiNong Gao (1)
  LiHui Li (1)
  WeiHua Liu (1)
  
• 关键词：wheat ; Agropyron cristatum addition line ; P chromosome ; Ph gene ; homoeologous chromosome synapsis
• 刊名：Chinese Science Bulletin
• 出版年：2010
• 出版时间：January 2010
• 年：2010
• 卷：55
• 期：2
• 页码：153-157
• 全文大小：2105KB
• 参考文献：1. Okamoto M. Asynaptic effect of chromosome V. Wheat Information Service, 1957, 5: 6-
  2. Riley R, Chapman V. Genetic control of the cytologically diploid behaviour of hexaploid wheat. Nature, 1958, 182: 713-15 CrossRef
  3. Mcintosh R A, Hart G E, Gale M D. Catalogue of gene symbols for wheat. Proceedings of the 8th Int ernational Wheat Genetics Symposium, July 20-5, 1993. Beijing: China Agricultural Scientech Press, 1993. 1366
  4. Mello-Sampayo T. Promotion of homologous pairing in hybrids of / Triticum aestivum × / Aegilops longissima. Genet Iber, 1971, 23: 1-
  5. Chen Q, Jahier J, Cauderon Y. Production and cytogenetical studies of hybrids between / Triticum aestivum (L)Thell and / Agropyron cristatum (L) faertn. C R Acad Sci III-Vie, 1989, 308: 425-30
  6. Li L H, Dong Y S. Production and cytogenetic study of intergeneric hybrids between / Triticum aestivum L. and / Agropyron desertorum (Fisch.) Schult (in Chinese). Sci China Ser B, 1990, 20: 492-96
  7. Li L H, Dong Y S, Zhou R H, et al. Cytogenetics and self-fertility of hybrids between / Triticum aestivum L. and / Agropyron cristatum (L.) Gaertn. (in Chinese). Chinese J Genet, 1995, 22: 109-14
  8. Ahmad F, Comeau A. A new intergeneric hybrid between / Triticum aestivum L. and / Agropyron fragile (Roth)Candargy: Variation in / A.fragile for suppression of the wheat / Ph-locus activity. Plant Breed, 1991, 106: 275-83 CrossRef
  9. Chen Q, Jahier J, Cauderon Y. Intergeneric hybrids between / Triticum aestivum and three crested wheatgrasses: / Agropyron mongolicum, A. michnoi and / A.desertorum. Genome, 1990, 33: 663-67
  10. Jauhar P P. Chromosome pairing in hybrids between hexaploid bread wheat and tetraploid crested wheatgrass ( / Agropyron cristatum). Hereditas, 1992, 116: 107-09 CrossRef
  11. Li L H, Dong Y S. Hybridization between / Triticum aestivum L. and / Agropyron michnoi Roshev.1. Production and cytogenetic study of F1 hybrids. Theor Appl Genet, 1991, 81: 312-16 CrossRef
  12. Limin A E, Fowler D B. An interspecific hybrid and amphiploid produced from / Triticum aestivum crosses with / Agropyron cristatum and / Agropyron desertorum. Genome, 1990, 33: 581-84
  13. Li L H, Li X Q, Li P, et al. Establishment of wheat- / Agropyron cristatum addition lines I. Cytology of F₃, F₂BC₁, BC₄ and BC3F1(in Chinese). Chinese J Genet, 1997, 24: 154-59
  14. Li L H, Yang X M, Zhou R H, et al. Establishment of wheat- / Agropyron cristatum addition lines II. Identification of alien chromosomes and analysis of development approaches (in Chinese). Chinese J Genet, 1998, 25: 538-44
  15. Friebe B, Jiang J, Gill B S, et al. Radiation-induced nonhomoeologous wheat- / Agropyron intermedium chromosomal translocations conferring resistance to leaf rust. Theor Appl Genet, 1993, 86: 141-49 CrossRef
  16. Le H T, Armstrong K C, Miki B. Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol Biol Rep, 1989, 7: 150-58 CrossRef
  17. MuKai Y, Nakahara Y. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome, 1993, 36: 489-94 CrossRef
  18. Pedersen C, Langridge P. Identification of the entire chromosome complement of bread wheat by two-colour FISH. Genome, 1997, 40: 589-93 CrossRef
  19. Segal G, Liu B, Vega J M, et al. Identification of a chromosome-specific probe that maps within the / Ph1 deletions in common and durum wheat. Theor Appl Genet, 1997, 94: 968-70 CrossRef
  20. Griffiths S, Sharp R, Foote T N, et al. Molecular characterization of / Ph1 as a major chromosome pairing locus in polyploid wheat. Nature, 2006, 439: 749-52 CrossRef
  21. Li L H, Dong Y S. Production and cytogenetic study of intergeneric hybrids between / Triticum aestivum and / Agropyron desertorum. Sci China Ser B, 1991, 34: 45-1
  22. Jubault M, Tanguy A M, Abélard P, et al. Attempts to induce homoeologous pairing between wheat and / Agropyron cristatum genomes. Genome, 2006, 49: 190-93
  
• 作者单位：GuoHui Yang (1)
  XinMing Yang (1)
  RuiHui Wang (1)
  AiNong Gao (1)
  LiHui Li (1)
  WeiHua Liu (1)
  
  1. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
  
• ISSN：1861-9541

文摘

P chromosomes may carry a genetic system that inhibits the Ph gene in wheat. Abnormal chromosome synapsis in wheat-Agropyron cristatum addition line II-21-2 (additional 1·4 recombinant P chromosome) was observed in this study. The results of cytogenetics and Ph1 gene amplification showed that the Ph1 gene was normal and the average number of quadrivalents or hexavalents was determined to be 0.41 and 0.13, respectively, in pollen-mother cells of wheat-Agropyron cristatum addition line II-21-2. The analysis of dual-color GISH/FISH showed that the P chromosomes were not directly involved in the composition of multivalents but could inhibit the effect of the Ph gene, leading to synapsis of wheat homoeologous chromosomes and translocation between wheat homoeologous chromosomes such as 3B-3D chromosomes. The characteristic of P chromosomes-promoting synapsis of wheat homoeologous chromosomes may have potential application in the genetic improvement of wheat.